Team PARIETTI
Pre-Clinical Investigation Platform (PIPC)Learn more about the platform
The main mission of the Pre-Clinical Investigation Platform is to meet the animal experimentation needs related to the scientific activity of the IRSL, in compliance with the applicable national and European regulations on the protection of animals used for scientific purposes (Directive 2010/63; Decree 2013-118). Particular attention is paid to animal welfare, through the establishment of an animal welfare structure and affiliation with an animal experimentation ethics committee.
The platform houses mice under controlled health conditions (SOPF – specific opportunistic pathogen free) and comprises two sectors: breeding and experimentation. This enables the housing and handling of transgenic mice (class 1 GMOs), including immunodeficient mouse lines for the establishment of xenografts.
The platform is notably equipped with small animal imaging systems: a bioluminescence/fluorescence imaging system and a two-photon microscope (with a gateway to the Technological Platform). These instruments allow better characterization of the pre-clinical models developed within the platform.
Use of the platform requires that a project authorization request has been submitted to the Ministry of Research, in agreement with the platform manager.
Services & Equipment
Services
Training
Practical training in technical procedures, development of a training plan and validation of technical skills.
Assistance
Support with drafting project authorization requests, and assistance with the execution of experimental procedures.
Zootechnics
Provision of scientific equipment for carrying out experimental procedures, and mouse breeding.
Equipment
Anesthesia
Isoflurane gas anesthesia stations: compact module with TEC3 evaporator (Minerve) equipped with an induction chamber and masks with thermoregulated supports.
Blood Analysis
Murine blood cell analysis: MS9-5 (Melet Schloesing Laboratoires).
In Vivo Small Animal Imaging
- Bioluminescence/fluorescence imager: IVIS Lumina III (Revvity)
- Two-photon microscope: LSM780 BIPHOTON (Zeiss)
Irradiator
X-ray generator: X-RAD320 (Actemium)
